DiNozzo’s last episode in the NCIS series was titled “Family First.” This episode aired as the finale of the thirteenth season on May 17, 2016. However, it is important to note that Michael Weatherly, the actor who portrayed DiNozzo, actually made his final on-screen appearance in the penultimate episode of the season, titled “Dead Letter,” which aired on May 10, 2016.
In “Family First,” DiNozzo decides to leave the team and NCIS after discovering that he has a daughter, Tali, with his former partner, Ziva David. Ziva had died in a mortar attack several years prior, but her death had remained a secret until this point. DiNozzo leaves NCIS to dedicate himself to raising Tali and protecting her from potential threats.
This departure marked a significant moment for both the character of DiNozzo and for the show itself. DiNozzo had been a central character on NCIS since its inception in 2003, and his departure left a void that would be filled by new characters in subsequent seasons.
It is worth mentioning that while DiNozzo left the NCIS team, the character has been referenced and mentioned in later seasons, and Weatherly even made a guest appearance in season 17. However, his last regular appearance as DiNozzo was in “Family First.”