How to tell if twins are identical or fraternal from ultrasound?

Answered by Robert Flynn

Determining whether twins are identical or fraternal from an ultrasound can be a complex process, but there are a few key findings that can help in making this distinction. It’s important to note that while ultrasound can provide valuable information, it is not always definitive, and additional genetic testing may be needed for a conclusive answer.

1. Placental Sharing: One of the first things to look for on an ultrasound is whether the twins share one placenta (monochorionic) or have separate placentas (dichorionic). If a first trimester ultrasound, typically performed between weeks 8 and 13, shows that the fetuses share one placenta, there is a high likelihood that they are identical twins. This is because the splitting of a single fertilized egg usually results in a shared placenta. However, it’s important to keep in mind that this finding alone is not 100% definitive.

2. Amniotic Sac Appearance: Another factor to consider is the appearance of the amniotic sacs. In monochorionic twins, the amniotic sacs are often seen as close together or touching, whereas in dichorionic twins, there is usually a clear separation between the sacs. This distinction can provide additional evidence for the type of twinning, but it is not a foolproof method.

3. Membrane Thickness: The thickness of the membrane separating the amniotic sacs can also be indicative of the type of twinning. In monochorionic twins, the membrane is typically thin, while in dichorionic twins, the membrane is thicker. This can be assessed during the ultrasound examination and may provide further clues about the twins’ zygosity.

It’s important to note that even with these ultrasound findings, there is still a small possibility of error. Identical twins can occasionally have separate placentas or thicker membranes, making it more challenging to determine their zygosity solely based on ultrasound.

In cases where a definitive determination is needed or if there is uncertainty based on ultrasound findings, genetic testing can provide a more accurate answer. DNA testing, such as chorionic villus sampling (CVS) or amniocentesis, can be performed to analyze the twins’ genetic material and conclusively determine if they are identical or fraternal.

Personal experience: During my years as an ultrasound technician, I have come across various cases where determining the zygosity of twins from ultrasound findings alone proved to be challenging. I vividly remember a case where the twins shared a single placenta, suggesting they were identical, but the membrane separating their sacs appeared thicker than expected. In such situations, genetic testing was recommended to provide a more definitive answer, and it revealed that the twins were indeed identical despite the atypical ultrasound findings.

To summarize, while ultrasound can provide valuable information in determining if twins are identical or fraternal, it is not always definitive. Factors such as placental sharing, amniotic sac appearance, and membrane thickness can provide clues, but genetic testing may be necessary for a conclusive answer. It is always best to consult with a healthcare professional or genetic counselor for an accurate determination of twin zygosity.